Yijian Gong, Manuel Murbach, Teruo Onishi, Myles Capstick, Toshio Nojima, and Niels Kuster, IEICE Transactions on Communications, Volume E97-B, Issue 3, pp. 631-637, online March 1, 2014
In this paper, we investigate the methods for assessing the impact of electromagnetic (EM) radiation on cells cultured for fertility research in monolayer and in suspension. The techniques used to culture spermatozoa in two layers, composed of culture medium and mineral oil, require adjustment of the dosimetric assessment routine. Exposure conditions in 35 mm Petri dishes were analyzed for three suspension-culturing configurations – medium only and two oil/medium multilayer configurations – and one oil/medium multilayer monolayer configuration. Detailed numerical and experimental dosimetry assessments were performed for the configurations exposed to the standing wave of R18 waveguides operated at 1950 MHz. The numerical dosimetry was verified by dosimetric temperature measurements. The non-uniformity of the specific absorption rate (SAR) distribution is 30% for monolayers, and 59 – 75% for suspension configurations.
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